View Bulletin. Learn More About Library Prep. Learn More About Data Analysis. DNA Isolation. Library Prep. Data Analysis. Your items have been added to the cart. Continue Shopping Go to Cart. Guided NGS Tutorials. Learn what to expect with these tips for each step of the NGS workflow. View Tutorials. Additional Resources. Start Course. This fluorescence is detected by a camera and recorded on a computer. Each of the terminator bases A, C, G and T give off a different colour. The fluorescently-labelled terminator group is then removed from the first base and the next fluorescently-labelled terminator base can be added alongside.
And so the process continues until millions of clusters have been sequenced. The DNA sequence is analysed base-by-base during Illumina sequencing, making it a highly accurate method. The sequence generated can then be aligned to a reference sequence, this looks for matches or changes in the sequenced DNA. Related Content:. What is DNA? What is a genome? What is DNA sequencing?
DNA Sequencing. Sequencing at Speed flash version. Uses of Deep Sequencing. Factors Impacting Cancer Sequencing Depth. Purity of the tumor Tumors usually consist of a mixture of normal and tumor tissue. Heterogeneity of the tumor Advanced tumors are frequently polyclonal.
Deep Sequencing for Bacterial Drug Resistance Studies A targeted deep sequencing assay identifies multidrug-resistant tuberculosis strains responsible for silent outbreaks.
Read Article. Featured Products. Additional Information. Broad Dynamic Range for Expression Profiling. In-Depth NGS Introduction This detailed overview of Illumina sequencing describes the evolution of genomic science, major advances in sequencing technology, key methods, the basics of Illumina sequencing chemistry, and more.
Read Introduction. See how scientists utilize NGS to make breakthrough discoveries. Read Article. Exploring the Tumor Microenvironment Researchers use single-cell techniques to study cancer microenvironments, to elucidate gene expression patterns and gain insights into drug resistance and metastasis. Using NGS to Study Rare Diseases Whole-exome and transcriptome sequencing prove beneficial in uncovering mutations and pathways associated with rare genetic diseases.
Evolution of Illumina NGS. NGS Experimental Considerations Learn about read length, coverage, quality scores, and other experimental considerations to help you plan your sequencing run.
Key Terms in NGS. Methods Guide Access the information you need—from BeadChips to library preparation for genome, transcriptome, or epigenome studies to sequencer selection, analysis, and support—all in one place. Access Guide. Genomics News. Interested in receiving newsletters, case studies, and information from Illumina based on your area of interest? Sign up now.
Related Solutions.
0コメント